Koirala, T.R., Hayashi, K., Chen, H-L., Ino, H., Kariya, N., Yanai, H., Choudhury, C.R. and Agaki, T., 1997. Malignant Lymphoma Induction of Rabbits with Oral Spray of Epstein-Barr Virus-related Herpesvirus from Si-IIA Cells (STLV-II-transformed cynomolus cell line): A Possible Animal Model for Epstein-Barr Virus Infection and subsequent virus-related tumors in humans. Pathology International, 47 (7), pp. 442-448.
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Malignant lymphoma (ML) was Induced in eight of nine rabbb inoculated by oral spray of the cell-free pellets from SI-IIA culture (MLV-ll-transformed leukocyte cell line of the Cynomolgus-producing Epsteln-Barr virus (EBV)-related herpesvirus) after 64–141 days. None of the rabbits inoculated with EBV from B-95–8 cells or HTLV-II from MOT cells developed ML. Malignant lymphomas were usually of diffuse, large-cell or mixed type. HTLV-II infection was excluded by the polymerase chain reaction (PCR) and the particle agglutination test. EBV-encoded RNA-1 and EBV-related DNA were detected in the tumor tissues by In situ hybridization and PCR, respectively. Anti-viral capsid antigen of EBV antibody (anti-VCA) was observed 3 weeks after oral inoculation of Si-IIA cell-free pellets. Polymerase chain reaction revealed continuous detection of EBV-related virus DNA in the peripheral blood leukocytes from 3 days after oral inoculation. These results show that ML induced orally wtth SI-IIA cell-free pellets was caused by EBV-related herpesvirus harbored by Si-IIA cells. Oral spray of EBV from B-95–8 also induced EBV Infection in rabbits, which was confirmed both by the presence of anti-VCA and by PCR. These oral infection and mallgnant lymphoma induction systems of rabbit using EBV-related virus from Si-IIA or human EBV are useful animal models for the study of EBV infection and EBV-related lymphomas in humans.
|Uncontrolled Keywords:||Epstein-Barr virus; Human T lymphotropic virus; Herpesvirus; Malignant lymphoma; Animal model|
|Subjects:||Technology > Medicine and Health|
|Group:||School of Health and Social Care > Centre for Postgraduate Medical Research and Education|
|Deposited By:||Mrs Jean Harris|
|Deposited On:||15 Jun 2009 12:59|
|Last Modified:||07 Mar 2013 15:09|
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