Chen, H-L., Hayashi, K., Koirala, T.R., Ino, H., Fuimoto, Y., Yoshikawa, Y., Choudhury, C.R. and Agaki, T., 1997. Malignant Lymphoma Induction in Rabbits by Oral Inoculation of Crude Virus Fraction prepared from T –B cells (Cynomol B-lymphoblastoid Cells harboring Epstein-Barr Virus related Simion Herpesvirus. Acta Medica Okayama, 51 (3), pp. 141-147.
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Official URL: http://escholarship.lib.okayama-u.ac.jp/amo/vol51/...
Malignant lymphoma was induced in Japanese (JWY), New Zealand (NZY) and Dutch (DUY) white rabbits by oral spray of cell-free pellets of culture fluid (crude virus fraction) of Ts-B6 cells (cynomolgus monkey B-lymphoblastoid cells harboring Epstein Barr virus-related simian herpesvirus or Cyno-EBV). Nine of 11 inoculated rabbits developed malignant lymphomas within 42-160 days after oral inoculation (JWY, 2/3; NZY, 5/6; DUY, 2/2). In contrast, none of the control rabbits inoculated in the same fashion with B95-8 (EBV-producing marmoset cell line) cell-free pellets developed malignant lymphoma. Most rabbits showed increased anti-VCA IgG and anti-EA-DR IgG antibody titers after inoculation by oral spray of Ts-B6 cell-free pellets. EBV-encoded RNA-1 was revealed in the tumor cells by in situ hybridization. EBV DNA was detected in the rabbit peripheral blood leukocytes (PBL) by polymerase chain reaction; the earliest positive result was obtained only two days after oral inoculation. These data suggest that orally administered Cyno-EBV in Ts-B6 cells infects PBL and then induces malignant lymphoma in rabbits. The availability of this animal model promises to clarify the role of EBV in human lymphoma and provides a means for studying prophylactic and therapeutic regimens.
|Uncontrolled Keywords:||Epstein-Barr virus; Malignant lymphoma; Oral inoculation; Simian herpesvirus; Animal model|
|Subjects:||Technology > Medicine and Health|
|Group:||School of Health and Social Care > Centre for Postgraduate Medical Research and Education|
|Deposited By:||Mrs Jean Harris|
|Deposited On:||15 Jun 2009 13:09|
|Last Modified:||07 Mar 2013 15:09|
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