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Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3/7.

Ruocco, N., Varella, S, Romano, G., Ianora, A., Bentley, M.G., Somma, D, Leonardi, A, Mellone, S, Zuppa, A and Costantini, M., 2016. Diatom-derived oxylipins induce cell death in sea urchin embryos activating caspase-8 and caspase 3/7. Aquatic Toxicology, 176, 128 - 140.

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Ruocco et al (2016) Aquatic Toxicology..pdf - Published Version
Available under License Creative Commons Attribution.


DOI: 10.1016/j.aquatox.2016.04.012


Diatoms are an important class of unicellular algae that produce bioactive secondary metabolites withcytotoxic activity collectively termed oxylipins, including polyunsaturated aldehydes (PUAs), hydroxy-acids (HEPEs), oxo-acids and epoxyalcohols. Previous results showed that at higher concentrations, thePUA decadienal induced apoptosis on copepods and sea urchin embryos via caspase-3 activation; atlower concentrations decadienal affected the expression levels of the caspase-8 gene in embryos of thesea urchin Paracentrotus lividus. In the present work, we studied the effects of other common oxylip-ins produced by diatoms: two PUAs (heptadienal and octadienal) and four hydroxyacids (5-, 9- 11- and15-HEPE) on P. lividus cell death and caspase activities. Our results showed that (i) at higher concentra-tions PUAs and HEPEs induced apoptosis in sea urchin embryos, detected by microscopic observationand through the activation of caspase-3/7 and caspase-8 measured by luminescent assays; (ii) at lowconcentrations, PUAs and HEPEs affected the expression levels of caspase-8 and caspase-3/7 (isolated forthe first time here in P. lividus) genes, detected by Real Time qPCR. These findings have interesting impli-cations from the ecological point of view, given the importance of diatom blooms in nutrient-rich aquaticenvironments.

Item Type:Article
Uncontrolled Keywords:Apoptosis; Caspases; Diatoms; Sea urchin
Group:Faculty of Science & Technology
ID Code:23515
Deposited By: Symplectic RT2
Deposited On:04 May 2016 13:43
Last Modified:14 Mar 2022 13:56


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