Klf15 Is Critical for the Development and Differentiation of Drosophila Nephrocytes.

Ivy, J.R., Catterson, J.H., Drechsler, M., Bodmer, R., Ocorr, K., Paululat, A. and Hartley, P. S., 2015. Klf15 Is Critical for the Development and Differentiation of Drosophila Nephrocytes. PLoS One, 10 (8), e0134620.

Full text available as:

[img]
Preview
PDF (OPEN ACCESS ARTICLE)
Published Version Klf15 Nephrocytes 2015.pdf - Published Version

13MB

DOI: 10.1371/journal.pone.0134620

Abstract

Insect nephrocytes are highly endocytic scavenger cells that represent the only invertebrate model for the study of human kidney podocytes. Despite their importance, nephrocyte development is largely uncharacterised. This work tested whether the insect ortholog of mammalian Kidney Krüppel-Like Factor (Klf15), a transcription factor required for mammalian podocyte differentiation, was required for insect nephrocyte development. It was found that expression of Drosophila Klf15 (dKlf15, previously known as Bteb2) was restricted to the only two nephrocyte populations in Drosophila, the garland cells and pericardial nephrocytes. Loss of dKlf15 function led to attrition of both nephrocyte populations and sensitised larvae to the xenotoxin silver nitrate. Although pericardial nephrocytes in dKlf15 loss of function mutants were specified during embryogenesis, they failed to express the slit diaphragm gene sticks and stones and did not form slit diaphragms. Conditional silencing of dKlf15 in adults led to reduced surface expression of the endocytic receptor Amnionless and loss of in vivo scavenger function. Over-expression of dKlf15 increased nephrocyte numbers and rescued age-dependent decline in nephrocyte function. The data place dKlf15 upstream of sns and Amnionless in a nephrocyte-restricted differentiation pathway and suggest dKlf15 expression is both necessary and sufficient to sustain nephrocyte differentiation. These findings explain the physiological relevance of dKlf15 in Drosophila and imply that the role of KLF15 in human podocytes is evolutionarily conserved

Item Type:Article
ISSN:1932-6203
Uncontrolled Keywords:Kindey ; Podocyte ; Drosophila ; KLF15 ; Development ; Genetics ; Cardiovascular ; Ageing
Subjects:UNSPECIFIED
Group:Faculty of Science and Technology
ID Code:22377
Deposited By: Unnamed user with email symplectic@symplectic
Deposited On:01 Sep 2015 11:31
Last Modified:01 Sep 2015 11:31

Downloads

Downloads per month over past year

More statistics for this item...
Repository Staff Only -