Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens.

Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C. and Gilmore, B.F., 2015. Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens. Marine Drugs, 13 (6), 3581 - 3605.

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DOI: 10.3390/md13063581

Abstract

The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

Item Type:Article
ISSN:1660-3397
Uncontrolled Keywords:Halidrys siliquosa ; antibiofilm ; antimicrobial ; biodiscovery ; clinical strains ; extraction ; Anti-Bacterial Agents ; Bacteria ; Biofilms ; Humans ; Ireland ; Microbial Sensitivity Tests ; Microscopy, Confocal ; Phaeophyta
Subjects:UNSPECIFIED
Group:Faculty of Science & Technology
ID Code:24477
Deposited By: Unnamed user with email symplectic@symplectic
Deposited On:08 Aug 2016 14:58
Last Modified:08 Aug 2016 14:58

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