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ToolsChantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T., 2015. A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). Journal of Applied Ichthyology, 31 (S1), 89 - 93.
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DOI: 10.1111/jai.12735
Abstract
Optimal conditions of short-term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution (1: 3) and incubated for 1 h in acidic, pH 6.5 (AC group) or alkaline, pH 8.5 (AL group) conditions. Subsequently, the sperm of both groups was further diluted (1: 3) in the immobilizing solution and the pH was adjusted to pH 7.51 (±0.02) and 7.56 (±0.07) in the AC and AL group respectively. The samples were kept in sealed bags and stored at 4°C. Viability (%) and motility (%) was estimated in samples obtained daily for the next 4 days. The results indicate that even a brief exposure of sperm to acid acidic conditions prior to storage accelerated the decline of sperm viability and motility during refrigeration.
| Item Type: | Article |
|---|---|
| ISSN: | 0175-8659 |
| Additional Information: | Special Issue: Proceedings of the 4th International Workshop on the Biology of Fish Gametes, 17-20 September, 2013, Albufeira, Algarve, Portugal |
| Group: | Faculty of Science & Technology |
| ID Code: | 25008 |
| Deposited By: | Symplectic RT2 |
| Deposited On: | 29 Nov 2016 15:59 |
| Last Modified: | 14 Mar 2022 14:00 |
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A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). (deposited 19 Oct 2015 09:50)
- A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). (deposited 29 Nov 2016 15:59) [Currently Displayed]
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