A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758).

Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T., 2015. A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). Journal of Applied Ichthyology, 31 (S1), 89 - 93.

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DOI: 10.1111/jai.12735

Abstract

Optimal conditions of short-term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution (1: 3) and incubated for 1 h in acidic, pH 6.5 (AC group) or alkaline, pH 8.5 (AL group) conditions. Subsequently, the sperm of both groups was further diluted (1: 3) in the immobilizing solution and the pH was adjusted to pH 7.51 (±0.02) and 7.56 (±0.07) in the AC and AL group respectively. The samples were kept in sealed bags and stored at 4°C. Viability (%) and motility (%) was estimated in samples obtained daily for the next 4 days. The results indicate that even a brief exposure of sperm to acid acidic conditions prior to storage accelerated the decline of sperm viability and motility during refrigeration.

Item Type:Article
ISSN:0175-8659
Additional Information:Special Issue: Proceedings of the 4th International Workshop on the Biology of Fish Gametes, 17-20 September, 2013, Albufeira, Algarve, Portugal
Subjects:UNSPECIFIED
Group:Faculty of Science & Technology
ID Code:25008
Deposited By: Unnamed user with email symplectic@symplectic
Deposited On:29 Nov 2016 15:59
Last Modified:29 Nov 2016 15:59

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