Production of live larvae following in vitro maturation of zebrafish oocytes.

Silva, L.A., Marques, L.S, Zhang, T., Rodrigues, R.B., Eloy, L.R. and Streit Jr., D.P., 2018. Production of live larvae following in vitro maturation of zebrafish oocytes. Theriogenology, 105, 115 - 119.

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DOI: 10.1016/j.theriogenology.2017.09.021

Abstract

This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.

Item Type:Article
ISSN:0093-691X
Uncontrolled Keywords:Carp pituitary ; Danio rerio ; In vitro maturation ; Ovarian follicles ; Pituitary gonadotropins
Group:Faculty of Science & Technology
ID Code:29863
Deposited By: Unnamed user with email symplectic@symplectic
Deposited On:12 Oct 2017 15:46
Last Modified:12 Oct 2017 15:46

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