Drosophila pericardial nephrocyte ultrastructure changes during ageing.

Psathaki, O.E., Denhem, L., Hartley, P. S. and Paululat, A., 2018. Drosophila pericardial nephrocyte ultrastructure changes during ageing. Mechanisms of Ageing and Development, 173 (July), pp. 9-20.

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DOI: 10.1016/j.mad.2018.04.006

Abstract

Here we show that a labyrinth channel compartment and slit diaphragms, which are the histological structures enabling insect nephrocytes ultrafiltration, are established during embryogenesis first by the garland nephrocytes (GCNs). The later pericardial nephrocytes, which represent the majority of functional nephrocytes in larvae and adults, lack these characteristic features at the embryonic stage. During larval development, a subpopulation of the pericardial cells survives and matures into functional nephrocytes (PCNs) displaying a fully differentiated slit diaphragm and a labyrinth channel compartment. Likely the embryonic pericardial cells have primary functions other than ultrafiltration (e.g. in production and secretion of ECM constituents). We also show, for the first time, that PCNs in the adult fly undergo dramatic histological degeneration upon ageing. The slit diaphragms disappear, the labyrinth channel system degenerates and the lysosomal compartment becomes highly enriched with electron-dense material. When using nephrocytes as a model for genetic screening purposes or to investigate the specific role of genes involved in endocytosis, histological changes occurring upon ageing need to be taken into account when interpreting structural data.

Item Type:Article
ISSN:0047-6374
Uncontrolled Keywords:Renal system nephrology; Nephrocytes; Pericardial cells; Heart; Dorsal vessel; Endocytosis; Ageing; Drosophila; nephrocyte; slit diaphragm; ultrastructure; pericardial cells; heart; autophagy; endocytosis; scavenger cells; podocytes
Group:Faculty of Science & Technology
ID Code:30609
Deposited By: Unnamed user with email symplectic@symplectic
Deposited On:26 Apr 2018 14:07
Last Modified:14 May 2018 11:47

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