Skip to main content

Biomimetic Properties of Force-spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application.

McColgan-Bannon, K. I. S., Upson, S., Gentile, P., Tausif, M., Russell, S., Dalgarno, K. and Ferreira, A. M., 2019. Biomimetic Properties of Force-spun PHBV Membranes Functionalised with Collagen as Substrates for Biomedical Application. Coatings, 9 (6), 350.

Full text available as:

[img]
Preview
PDF (OPEN ACCESS ARTICLE)
Biomimetic.pdf - Published Version
Available under License Creative Commons Attribution.

2MB

DOI: 10.3390/coatings9060350

Abstract

The force-spinning process parameters (i.e., spin speed, spinneret-collector distance, and polymer concentration), optimised and characterised in previous work by this group, allowed the rapid fabrication of large quantities of high surface area poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) polymeric fibre membranes. This paper examined the potential application for force-spun PHBV fibres functionalised with type I collagen for tissue regeneration applications. PHBV fibre scaffolds provide a biologically suitable substrate to guide the regeneration of dermal tissues, however, have poor cellular adhesion properties. The grafting of collagen type-I to PHBV fibres demonstrated improved cell adhesion and growth in Neo-NHDF (neonatal human dermal fibroblasts) fibroblasts. The examination of fibre morphology, thermal properties, collagen content, and degradability was used to contrast the physicochemical properties of the PHBV and PHBV-Collagen fibres. Biodegradation models using phosphate buffered saline determined there was no appreciable change in mass over the course of 6 weeks; a Sirius Red assay was performed on degraded samples, showing no change in the quantity of collagen. Cell metabolism studies showed an increase in cell metabolism on conjugated samples after three and 7 days. In addition, in vitro cytocompatibility studies demonstrated superior cell activity and adhesion on conjugated samples over 7 days.

Item Type:Article
ISSN:2079-6412
Uncontrolled Keywords:PHBV; collagen; force spinning; functionalization; tissue engineering
Group:Faculty of Science & Technology
ID Code:35718
Deposited By: Unnamed user with email symplectic@symplectic
Deposited On:02 Jul 2021 14:04
Last Modified:02 Jul 2021 14:04

Downloads

Downloads per month over past year

More statistics for this item...
Repository Staff Only -