Marcotti, S., Sánchez-Sánchez, B. J., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M-D-C, Matsubayashi, Y. and Stramer, B. M., 2021. Protocol for intervention-free quantification of protein turnover rate by steady-state modeling. STAR Protocols, 2, 100377.
Full text available as:
|
PDF (OPEN ACCESS ARTICLE)
Protocol for intervention-free quantification of protein turnover rate by steady-state modeling.pdf - Published Version Available under License Creative Commons Attribution. 3MB | |
Copyright to original material in this document is with the original owner(s). Access to this content through BURO is granted on condition that you use it only for research, scholarly or other non-commercial purposes. If you wish to use it for any other purposes, you must contact BU via BURO@bournemouth.ac.uk. Any third party copyright material in this document remains the property of its respective owner(s). BU grants no licence for further use of that third party material. |
DOI: 10.1016/j.xpro.2021.100377
Abstract
Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This approach can be used to calculate production and degradation rates and to infer protein half-life. This model was successfully employed to quantify turnover during Drosophila embryogenesis, and we hypothesize that it will be applicable to diverse in vivo or in vitro systems.
Item Type: | Article |
---|---|
ISSN: | 2666-1667 |
Group: | Faculty of Science & Technology |
ID Code: | 38484 |
Deposited By: | Symplectic RT2 |
Deposited On: | 24 Jul 2023 14:37 |
Last Modified: | 24 Jul 2023 14:37 |
Downloads
Downloads per month over past year
Repository Staff Only - |