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Protocol for intervention-free quantification of protein turnover rate by steady-state modeling.

Marcotti, S., Sánchez-Sánchez, B. J., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M-D-C, Matsubayashi, Y. and Stramer, B. M., 2021. Protocol for intervention-free quantification of protein turnover rate by steady-state modeling. STAR Protocols, 2, 100377.

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DOI: 10.1016/j.xpro.2021.100377

Abstract

Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This approach can be used to calculate production and degradation rates and to infer protein half-life. This model was successfully employed to quantify turnover during Drosophila embryogenesis, and we hypothesize that it will be applicable to diverse in vivo or in vitro systems.

Item Type:Article
ISSN:2666-1667
Group:Faculty of Science & Technology
ID Code:38484
Deposited By: Symplectic RT2
Deposited On:24 Jul 2023 14:37
Last Modified:24 Jul 2023 14:37

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