Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T., 2015. A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). Journal of Applied Ichthyology, 31 (S1), 89 - 93 .
Full text available as:
jai12735 Chntzaropoulos et al 2015 carp sperm pH.pdf - Accepted Version
Optimal conditions of short-term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution (1: 3) and incubated for 1 h in acidic, pH 6.5 (AC group) or alkaline, pH 8.5 (AL group) conditions. Subsequently, the sperm of both groups was further diluted (1: 3) in the immobilizing solution and the pH was adjusted to pH 7.51 (±0.02) and 7.56 (±0.07) in the AC and AL group respectively. The samples were kept in sealed bags and stored at 4°C. Viability (%) and motility (%) was estimated in samples obtained daily for the next 4 days. The results indicate that even a brief exposure of sperm to acid acidic conditions prior to storage accelerated the decline of sperm viability and motility during refrigeration.
|Additional Information:||Special Issue: Proceedings of the 4th International Workshop on the Biology of Fish Gametes, 17-20 September, 2013, Albufeira, Algarve, Portugal|
|Group:||Faculty of Science and Technology|
|Deposited By:||Unnamed user with email symplectic@symplectic|
|Deposited On:||19 Oct 2015 09:50|
|Last Modified:||08 May 2016 01:08|
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